DNA sequencing
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A cloned fragment of DNA was sequenced using the dideoxy method. A part of the autoradiogram of the sequencing gel is represented here:ddAddCddTddCDeduce the nucleotide sequence of the DNA nucleotide chain synthesized from the primer. Label the 5' and 3' ends. Deduce the nucleotide sequence of the DNA nucleotide chain used as the template strand. Label the 5' and 3' ends. What is the difference between Sanger sequencing and new generation sequencing (NGS)?
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